Recombinant E-cadherin antibody (full length)

Catalog No. ABIN7470613

The Rabbit Monoclonal anti-E-cadherin antibody (Clone 035R) (ABIN7470613) specifically detects E-cadherin in IHC. The antibody is reactive with Human samples.

Quick Overview for Recombinant E-cadherin antibody (full length) (ABIN7470613)

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This E-cadherin antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: 035R

Product Details anti-E-cadherin Antibody

Binding Specificity: full length

Purpose: E-Cadherin (035R)

Immunogen: Recombinant full-length human E-Cadherin protein

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: In the liver, at least a moderate membranous staining should be seen in hepatocytes while bile duct epithelia stain strongly. In the colon, only epithelium should display a strong membranous staining reaction.

Product Description: E-Cadherin antibody validated for immunohistochemistry on 76 different Normal Tissues

Negative Control: Tonsil or appendix: No staining reaction must be seen in stromal cells such as lymphocytes, plasma cells, smooth muscle cells or endothelial cells.

Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 ° C. Antibody is stable for 24 months. Non- hazardous.

Expiry Date: 24 months

Target Details for E-cadherin

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Alternative Name: E-Cadherin

Background: Gene Alias: Arc 1, cadherin 1 type 1 E-cadherin, Cadherin1, CAM 120/80, CD324, CDH1, CDHE, E-Cad/CTF3, E-cadherin, ECAD, Epithelial cadherin, epithelial calcium dependent adhesion protein, Liver cell adhesion molecule (LCAM), Uvomorulin (UVO)

UniProt: P12830

Pathways: WNT Signaling, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation