Recombinant Estrogen Receptor alpha antibody (AA 495-595)

Catalog No. ABIN7477471

This anti-Estrogen Receptor alpha antibody is a Rabbit Monoclonal antibody detecting Estrogen Receptor alpha in IHC. Suitable for Human.

Quick Overview for Recombinant Estrogen Receptor alpha antibody (AA 495-595) (ABIN7477471)

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Estrogen Receptor alpha antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-564R

Product Details anti-Estrogen Receptor alpha Antibody

Binding Specificity: AA 495-595

Immunogen: Recombinant fragment (around aa495-595) of human ER alpha protein (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Uterine cervix: virtually all squamous and endocervical cells must show a moderate to strong nuclear staining. Tonsil: Scattered follicular dendritic cells in germinal centers and some squamous epithelial cells in tonsil crypts must show an at least weak nuclear ER immunostaining.

Negative Control: Tonsil: B-cells in mantle zones and within germinal centers must be negative.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for Estrogen Receptor alpha

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Alternative Name: Estrogen Receptor

Background: Estrogen Receptor alpha delta 4*5,6,7*/654 isoform,Estrogen Receptor alpha delta 4 +49 isoform, Nuclear receptor subfamily 3 group A member 1,Estrogen Receptor antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P03372

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly