Recombinant TNF alpha antibody

Catalog No. ABIN7540552

Supplier Product No.: 400-001-mt3

The Humanized Monoclonal anti-TNF alpha antibody (Clone Abz520) (ABIN7540552) specifically detects TNF alpha in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for Recombinant TNF alpha antibody (ABIN7540552)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Antibody Type: Recombinant Antibody

Fragment: Fab fragment

Reactivity: Human

Host: Humanized

Clonality: Monoclonal

Conjugate: This TNF alpha antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: Abz520

Product Details anti-TNF alpha Antibody

Supplier Product No.: 400-001-mt3

Supplier: Rockland

Purpose: Recombinant Anti-TNF alpha Fab Antibody

Purification: Humanized Recombinant TNFα Fab fragment was expressed in CHO cells.

Purity: > 95 % by SDS-PAGE analysis

Sterility: Sterile filtered

Isotype: IgG1

Application Details

Application Notes:

ELISA_Dilution: User Optimized

Western_Blot_Dilution: User Optimized

Comment:

Humanized Recombinant Anti-Anti-TNFα Fab fragment Antibody has been tested for use in Western Blot and ELISA. This antibody recognizes structured TNFα and will work in western blot when the protein has not been denatured with DTT or bMe. Although not tested, this antibody could be useful in in IHC and in in-vivo and other cellular assays. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 30 % Glycerol

Preservative: None

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store vial at -20° C. Dilute only prior to immediate use.

Expiry Date: 6 months

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF alpha

Background: APC1 antibody, Cachectin antibody, DIF antibody, Differentiation inducing factor antibody, Macrophage cytotoxic factor antibody, MCF antibody, Necrosin antibody, Tumour Necrosis Factor Alpha antibody, rabbit anti-Tumor Necrosis Factor Alpha Antibody, rabbit anti-TNF Alpha Antibody, Tumor necrosis factor, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Tumor necrosis factor membrane form, N-terminal fragment, NTF, Intracellular domain 1, ICD1, Intracellular domain 2, ICD2, C-domain 1, C-domain 2, Tumor necrosis factor, soluble form, TNF, TNFA, TNFSF2,Anti-TNF alpha Antibody recognizes TNF alpha (TNF, cachexin, cachectin, tumor necrosis factor-alpha or TNF-α) a cytokine involved in systemic inflammation. TNF alpha is a member of a group of cytokines that stimulate the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by other cell types as well. The primary role of TNF alpha is in the regulation of immune cells. TNF is an endogenous pyrogen that is able to induce fever, apoptotic cell death, sepsis (through IL-1 & IL-6 production), cachexia, inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNF production has been implicated in a variety of human diseases, including Alzheimer's disease, cancer, major depression, and inflammatory bowel disease (IBD). Anti-TNF alpha antibody, Adalimumab, a monoclonal antibody, working by inactivating TNFα is approved to treat rheumatoid arthritis, psoriatic arthritis, ankylosing spondylitis, Crohn's disease, ulcerative colitis, psoriasis, hidradenitis suppurativa, uveitis, and juvenile idiopathic arthritis and sold under the brand name Humira. This antibody is made from a Adalimumab Fab fragment making it specific, efficient, and effective. Humanized Recombinant Anti-TNFα Fab fragment Antibody is useful for researchers interested in Cancer and Inflammatory disease research.

Gene ID: 7124

NCBI Accession: NP_000585

UniProt: P01375

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome