Recombinant CD4 antibody

Catalog No. ABIN7543050

The Mouse Monoclonal anti-CD4 antibody is suitable to detect CD4 in samples from Rat. It has been validated for FACS, WB, IP, SPR and Bind.

Quick Overview for Recombinant CD4 antibody (ABIN7543050)

Target: CD4

Antibody Type: Recombinant Antibody

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD4 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Surface Plasmon Resonance (SPR), Binding Studies (Bind)

Clone: OX-68

Product Details anti-CD4 Antibody

Purpose: Anti-CD4 [OX-68], Mouse IgG2a, kappa

Specificity:

This antibody is reactive with rat CD4 domain 3 & 4, and is partly competitive with OX-67 & 69.

Application Notes original Clone: This antibody binds to rat CD4. Upon challenge with HIV-1, this antibody was able to significantly inhibit viral entry into HeLa cells expressing a mutant of rat CD4 that binds gp120 (SImon, 1997). Similarly, pre-binding of gp120 resulted in a 50 % reduction in binding of this antibody, indicating epitope masking. Analogous effects on viral entry and synctium formation are observed with MAbs against human CD4.

Purification: Protein A affinity purified

Immunogen: This antibody was prepared by subcutaneously injecting BALB/c mice with a soluble form of rat CD4, consisting of domains 3 and 4. A boost of a mixture of free sCD4 and sCD4 bound to cells was administered intravenously.

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

OriginalSpeciesName: Mouse

OriginalFormat: IgG2a

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD4

Target: CD4

Alternative Name: CD4

Background: Leu3/T4, T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, W3/25 antigen

UniProt: P05540

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events