Recombinant CD47 antibody

Catalog No. ABIN7543268

This Rabbit Chimeric antibody specifically detects CD47 in FACS, ELISA, IP and BR. It exhibits reactivity toward Human.

Quick Overview for Recombinant CD47 antibody (ABIN7543268)

Target: CD47

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This CD47 antibody is un-conjugated

Application: Flow Cytometry (FACS), ELISA, Immunoprecipitation (IP), Blocking Reagent (BR)

Clone: B6H12

Product Details anti-CD47 Antibody

Purpose: Anti-CD47 [B6H12], Rabbit IgG, kappa

Specificity: This antibody binds the extracellular domain of the human CD47, which is broadly distributed on normal adult tissues, as well as ovarian tumors, and is especially abundant in some epithelia and the brain. IAP or integrin assocaited protein has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It further plays an important role in memory formation and synaptic plasticity in the hippocampus. It also acts as a receptor for SIRPA. Binding to SIRPA prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing BALB/c mice with purified placental RGD-binding proteins (PRBP).

Isotype: IgG kappa

Application Details

Application Notes: This antibody reacts with placental RGD-binding proteins and human neutrophils in an ELISA (PMID: 2785522). This antibody binds CD47 expressed on human neutrophils in a flow cytometric assay. This antibody immunoprecipitated vitronectin receptor (VnR) from detergent-solubilized placental membrane preparations (PMID: 2785522). It is a function-blocking antibody that inhibits CD47 interactions with TSP1, SIRPα, some integrins, and EGF receptor (PMID: 19636412, 10572074, 19004835, 26840086). This antibody directly induces killing of tumor cells by NK cells, B cell migratiom, and regulatory T cell differentiation (PMID: 18497546, 10891456, 16951312). B6H12 enhanced antibody-dependent cellular phagocytosis (ADCP) of human acute myeloid leukemia (AML) cells in vitro by human and mouse macrophages (PMID: 19632179). THis antibody also inhibited AML tumor growth in NOD/SCID/IL2Rγ- (NSG) mice by blocking this ""don't eat me"" signal (PMID: 19632179). B6H12 was used in several preclinical studies to explore combining CD47 blockade with therapeutic antibodies that target specific cancers. This antibody in combination with CD20 antibody rituximab, to promote phagocytosis in vitro and eradicated non-Hodgkin lymphoma in NSG mice (PMID: 20813259).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD47

Target: CD47

Alternative Name: CD47

Background: IAP, MER6, OV-3, Leukocyte surface antigen CD47, Antigenic surface determinant protein OA3, Integrin-associated protein, Protein MER6, ovarian cancer marker

UniProt: Q08722