Recombinant Keratin 5/6 antibody (AA 488-590)

Catalog No. ABIN7543312

The Mouse Monoclonal anti-Keratin 5/6 antibody (Clone AbM58019-7H4) (ABIN7543312) specifically detects Keratin 5/6 in IHC and ELISA. The antibody is reactive with Human samples.

Quick Overview for Recombinant Keratin 5/6 antibody (AA 488-590) (ABIN7543312)

Target: Keratin 5/6 (KRT5/6)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Keratin 5/6 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Clone: AbM58019-7H4

Product Details anti-Keratin 5/6 Antibody

Binding Specificity: AA 488-590

Purpose: Anti-Cytokeratin 5/6 [AbM58019-7H4], Mouse IgG2b, kappa

Specificity: This antibody is capable of binding human cytokeratin 5 and human cytokeratin 6. Cytokeratins are intermediate filaments found in the intracytoplasmic cytoskeleton of epithelial tissue. Cytokeratin 5/6 is often expressed in a basal cell layer of normal mammary ducts. Studies have also shown that CK5/6 can be used as a characteristic marker of basal-like breast cancer.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2b

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing 4-6 weeks old female ICR mice with recombinant protein comprising amino acids 488-590 of cytokeratin 5 in equal volume of Freund's incomplete adjuvant.

Isotype: IgG2b kappa

Application Details

Application Notes: The binding characterization of this antibody was done using ELISA. The original IgG2b antibody binds cytokeratin 5/6 with a high affinity constant of 3.84 x 10^(9) M^(-1). This antibody was also used in the immunohistochemical analysis of esophagus and esophageal squamous cell carcinoma. The immunohistochemical staining pattern of this antibody is similar to commercially available antibody D5/16B4 (CN113845592).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for Keratin 5/6

Target: Keratin 5/6 (KRT5/6)

Alternative Name: Cytokeratin 5/6

Background: CK5/6, CK5, CK-5, K5 KRT5, Keratin, type II cytoskeletal 5, cytokeratin-5, 58 kDa cytokeratin, Type-II keratin Kb5, CGMCC NO 21982, D5/16B4

UniProt: P13647