Recombinant Keratin 5/6 antibody (C-Term)

Catalog No. ABIN7795768

The Rabbit Monoclonal anti-Keratin 5/6 antibody (Clone BLR340M) (ABIN7795768) specifically detects Keratin 5/6 in WB and FACS. The antibody is reactive with Human samples.

Quick Overview for Recombinant Keratin 5/6 antibody (C-Term) (ABIN7795768)

Target: Keratin 5/6 (KRT5/6)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Keratin 5/6 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Clone: BLR340M

Product Details anti-Keratin 5/6 Antibody

Binding Specificity: C-Term

Purpose: Rabbit anti-Cytokeratin 5/6 Recombinant Monoclonal Antibody [BLR340M]

Purification: Purified

Immunogen: between 540 and 590 (C-term)

Isotype: IgG

Application Details

Application Notes: WB,11000,Flow-Cyt,Fixed in 4 % formaldehyde and permeabilized with 90 % methanol. 1 μL per 1 x 10^6 cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 40 μg/mL

Buffer: Phosphate Buffered Saline (PBS) with 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2 - 8 °C

Expiry Date: 12 months

Target Details for Keratin 5/6

Target: Keratin 5/6 (KRT5/6)

Alternative Name: Cytokeratin 5/6

Background: Cytokeratins 5 and 6 are type II cytokeratins and consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. In normal tissue, Cyokeratin 5 is specifically expressed in the basal layer of the epidermis with family member KRT14 while Cytokeratin 6 is expressed with family members KRT16 and/or KRT17 in the filiform papillae of the tongue, the stratified epithelial lining of oral mucosa and esophagus, the outer root sheath of hair follicles, and the glandular epithelia. Cytokeratins 5 and 6 are often referred to together as "CK5/6", and are commonly expressed in various cancers, particularly those originating from epithelial tissues, and their presence is often used as a marker to identify squamous cell carcinomas or tumors with a basal cell-like phenotype [taken from NCBI Entrez Gene (Gene ID: < a href="https://www.ncbi.nlm.nih.gov/gene/3852" class="gene-values" target="_blank">3852 and < a href="https://www.ncbi.nlm.nih.gov/gene/3853" class="gene-values" target="_blank">3853)].

UniProt: P02538, P13647