PCNA antibody (AA 200-250)

Catalog No. ABIN723006

This anti-PCNA antibody is a Mouse Monoclonal antibody detecting PCNA in WB, IHC (p) and IF (p). Suitable for Human, Mouse, Rat and Rabbit. This Primary Antibody has been cited in 4+ publications.

Quick Overview for PCNA antibody (AA 200-250) (ABIN723006)

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reactivity: Human, Mouse, Rat, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This PCNA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Clone: 1C11

Product Details anti-PCNA Antibody

Binding Specificity: AA 200-250

Cross-Reactivity: Human, Mouse, Rabbit, Rat

Purification: Purified by Protein G.

Immunogen: KLH conjugated synthetic peptide derived from human PCNA

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-PCNA antibody (ABIN723006)

Jiang, Zhang, Ding, Duan, Lu: "Inflammation and cancer: inhibiting the progression of residual hepatic VX2 carcinoma by anti-inflammatory drug after incomplete radiofrequency ablation." in: International journal of clinical and experimental pathology, Vol. 8, Issue 11, pp. 13945-56, (2016) (PubMed).

Zhang, Wu, Cao, Zhong, Zhou: "Generation of glucagon-like peptide-2-expressing Saccharomyces cerevisiae and its improvement of the intestinal health of weaned rats." in: Microbial biotechnology, Vol. 9, Issue 6, pp. 846-857, (2016) (PubMed).

Hao, Zhang, Cheng, Li, Sun, Zhang, Guo, Li: "Imidapril inhibits right ventricular remodeling induced by low ambient temperature in broiler chickens." in: Poultry science, Vol. 92, Issue 6, pp. 1492-7, (2013) (PubMed).

Chen, Shi, Shu, Xie, Yang, Zhang, Ruan, Lin, Lin, Shen, Zhang, Feng, Xie: "The effect of porcine ADM to improve the burn wound healing." in: International journal of clinical and experimental pathology, Vol. 6, Issue 11, pp. 2280-91, (2013) (PubMed).

Target Details for PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Alternative Name: PCNA

Background:

Synonyms: ATLD2, Proliferating cell nuclear antigen, PCNA, Cyclin

Background: Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.

Gene ID: 5111

UniProt: P12004

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy