TNF alpha antibody

Catalog No. ABIN7565960

The Rabbit Polyclonal anti-TNF alpha antibody is suitable to detect TNF alpha in samples from Human. It has been validated for WB, IHC, ELISA, IF and FM.

Quick Overview for TNF alpha antibody (ABIN7565960)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNF alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM)

Product Details anti-TNF alpha Antibody

Purpose: TNF alpha Antibody

Cross-Reactivity (Details): The antiserum is directed against mature 17,000 MW human TNFa and is useful in determining its presence in various assays.

Characteristics: Synonyms: APC1 antibody, Cachectin antibody, DIF antibody, Differentiation inducing factor antibody, Macrophage cytotoxic factor antibody, MCF antibody, Necrosin antibody, Tumour Necrosis Factor Alpha antibody, rabbit anti-Tumor Necrosis Factor Alpha Antibody, rabbit anti-TNF Alpha Antibody, Tumor necrosis factor, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Tumor necrosis factor membrane form, N-terminal fragment, NTF, Intracellular domain 1, ICD1, Intracellular domain 2, ICD2, C-domain 1, C-domain 2, Tumor necrosis factor, soluble form, TNF, TNFA, TNFSF2

Purification: This antiserum has been heated to 56°C for 30 minutes.

Sterility: Sterile filtered

Immunogen:

Immunogen: The whole rabbit serum was prepared by repeated immunizations with recombinant human TNFa produced in E.coli.

Immunogen Type: Recombinant Protein

Application Details

Application Notes: Application Note: Anti-Human TNFα has been tested for use in immunohistochemistry, immunofluorescence, and immunoblotting. It recognizes the 17,000 MW TNFα. Reactivity in other immunoassays is unknown. Immunohistochemistry Dilution: 1:100 - 1:500 Western Blot Dilution: 1:500 - 1:2,000 ELISA Dilution: 1:1,000 - 1:5,000 IF Microscopy Dilution: 1:100 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 75 mg/mL

Buffer:

Buffer: None

Stabilizer: None

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF

Background: Background: Anti TNF alpha Antibody recognizes TNF alpha (TNF, cachexin, cachectin, tumor necrosis factor-alpha or TNF-α) a cytokine involved in systemic inflammation. TNF alpha is a member of a group of cytokines that stimulate the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by other cell types as well. The primary role of TNF alpha is in the regulation of immune cells. TNF is an endogenous pyrogen that is able to induce fever, apoptotic cell death, sepsis (through IL-1 & IL-6 production), cachexia, inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNF production has been implicated in a variety of human diseases, including Alzheimer's disease, cancer, major depression, and inflammatory bowel disease (IBD).

Gene ID: 7124

UniProt: P01375

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome