TNF alpha antibody

Catalog No. ABIN7565991

The Rabbit Polyclonal anti-TNF alpha antibody (ABIN7565991) specifically detects TNF alpha in WB and ELISA. The antibody is reactive with Mouse samples.

Quick Overview for TNF alpha antibody (ABIN7565991)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNF alpha antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-TNF alpha Antibody

Purpose: TNF alpha Antibody

Cross-Reactivity (Details): The antibody is directed against mature 17,000 MW mouse TNFα and is useful in determining its presence in various assays.

Characteristics: Synonyms: rabbit anti-TNF alpha antibody, rabbit anti-TNFa antibody, rabbit anti-tumor necrosis factor alpha antibody, APC1 antibody, Cachectin antibody, DIF antibody, Differentiation inducing factor antibody, Macrophage cytotoxic factor antibody, MCF antibody, Necrosin antibody, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, TNFA

Purification: TNF alpha Antibody has been heated to 56°C for 30 minutes.

Sterility: Sterile filtered

Immunogen:

Immunogen: This antibody was produced from whole rabbit serum prepared by repeated immunizations with recombinant mouse TNFalpha produced in E.coli.

Immunogen Type: Recombinant Protein

Application Details

Application Notes: Application Note: This antibody against Mouse TNFα has been tested for use in immunoblotting.  This antibody is suitable for ELISA, immunohistochemistry, neutralizations, radioimmunoassay, and immunoprecipitation. It recognizes the 17,000 MW TNFα.  Reactivity in other immunoassays is unknown. This antibody will recognize the cell-bound precursor of TNFα as a 26,000 protein in immunoblots, particularly in denatured samples. This antibody is also useful for neutralization of mouse activity in bioassays. It does not neutralize the biological activity of lymphotoxin. For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended. Western Blot Dilution: 1:500 - 1:2,000 ELISA Dilution: 1:1,000 - 1:5,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: Tnf

Background: Background: TNF alpha Antibody detects TNF-a protein. TNF-a is a cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is a potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Anti-TNF-alpha Antibody is ideal for investigators involved in Cell Signaling, Immunology and Signal Transduction research.

Gene ID: 21926

UniProt: P06804

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome