Collagen Type I antibody

Catalog No. ABIN7566413

The Rabbit Polyclonal anti-Collagen Type I antibody has been validated for WB and ICC. It is suitable to detect Collagen Type I in samples from Human, Mouse, Rat, Cow, Dog, Sheep, Pig, Cat and Hamster.

Quick Overview for Collagen Type I antibody (ABIN7566413)

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Sheep, Pig, Cat, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Collagen Type I antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Product Details anti-Collagen Type I Antibody

Purpose: anti-Collagen Type 1 (3/4 fragment), pAb

Characteristics:

Polyclonal Antibody. Recognizes rat and bovine collagen type 1. Based on sequence identity should also recognize human, mouse, guinea pig, dog, cat, donkey, pig, cow, sheep and chicken collagen type 1. Source: Rabbit. Applications: ICC. Liquid. In PBS with 1 mg/mL BSA and 0.02 % sodium azide. The proteolysis of collagens plays an important role in numerous physiological and pathological situations such as morphogenesis, wound healing, arthritis, arteriosclerosis and tumor metastasis. Triple helical type I collagens are made up of two alpha1 (I) and one alpha2 (I) chains, and are found in skin, tendon, ligament and interstitial tissues. Due to their fibrillary structure native collagens are resistant to most proteases. They are substrates for certain matrix metalloproteinases (MMPs), which constitute a family of zinc-dependent enzymes catalyzing the degradation of extracellular matrix components. Initial MMP-8 dependent cleavage of collagen into the characteristic 3/4 and 1/4 fragments has been shown to enable MMP-9 diffusion along the protein helix with preferential binding to the collagen 3/4 fragment tail. Finally, untwisting of the helix end results in the local denaturation of the triple helical structure.

The proteolysis of collagens plays an important role in numerous physiological and pathological situations such as morphogenesis, wound healing, arthritis, arteriosclerosis and tumor metastasis. Triple helical type I collagens are made up of two alpha1 (I) and one alpha2 (I) chains, and are found in skin, tendon, ligament and interstitial tissues. Due to their fibrillary structure native collagens are resistant to most proteases. They are substrates for certain matrix metalloproteinases (MMPs), which constitute a family of zinc-dependent enzymes catalyzing the degradation of extracellular matrix components. Initial MMP-8 dependent cleavage of collagen into the characteristic 3/4 and 1/4 fragments has been shown to enable MMP-9 diffusion along the protein helix with preferential binding to the collagen 3/4 fragment tail. Finally, untwisting of the helix end results in the local denaturation of the triple helical structure.

Purification: Antigen affinity purified.

Immunogen: Synthetic peptide (human sequence) corresponding to the C-terminal end of the N-terminal three quarter collagen fragment (Col1 3/4).

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: In PBS with 1 mg/mL BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment:

+4°C

Stable for at least 1 year after receipt when stored at -20°C.

Target Details for Collagen Type I

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Alternative Name: Collagen Type 1