GFP antibody

Catalog No. ABIN7595317

This anti-GFP antibody is a Chicken Polyclonal antibody detecting GFP in WB and IHC. Suitable for Aequorea victoria.

Quick Overview for GFP antibody (ABIN7595317)

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Chicken

Clonality: Polyclonal

Conjugate: This GFP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-GFP Antibody

Purpose: Chicken antibody to EGFP/GFP/Venus

Specificity: Specific for EGFP/GFP/Venus

Cross-Reactivity (Details): Any organism expressing EGFP/GFP/Venus.

Purification: IgY purified on IgY column and by ammonium sulfate cut

Immunogen: Recombinant EGFP/GFP/Venus protein was used as the antigen.

Isotype: IgY

Application Details

Application Notes: IHC WB. A dilution of 1 : 1000 is recommended. The optimal dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 100 µL of DI sterile water. Centrifuge to remove any insoluble material.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: EGFP/GFP/Venus

Background: GFP is a naturally fluorescent protein discovered by chance in the 1960s by Shimomura et al. GFP makes green light out of aequorin's blue light. The chromophore is a modified Ser-Tyr-Gly sequence. GFP can emit light once this short tripeptide has adopted a cyclic conformation with the help of a oxygen. BIOPHYSICOCHEMICAL PROPERTIES: Excitation max (nm): 488, Emission max (nm): 509, Extinction coefficient (Cm-1M-1): 61000. Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer reporter of gene expression or as a measure of protein-protein interactions.