PPARG antibody (AA 209-477)

Catalog No. ABIN7596019

The Mouse Monoclonal anti-PPARG antibody is suitable to detect PPARG in samples from Human. It has been validated for WB, ELISA, ICC, FACS and IF.

Quick Overview for PPARG antibody (AA 209-477) (ABIN7596019)

Target: PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PPARG antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: AT4F5

Product Details anti-PPARG Antibody

Binding Specificity: AA 209-477

Purpose: Human PPAR gamma/NR1C3 antibody

Purification: Purified

Immunogen: Recombinant human PPARG (209-477aa) purified from E. coli.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate-Buffered Saline ( pH 7.4) with 0.02 % Sodium Azide, 10 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing cycles

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Target Details for PPARG

Target: PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))

Alternative Name: PPAR gamma/NR1C3

Background: PPAR gamma, also known as Peroxisome proliferator-activated receptor gamma, is member of the nuclear hormone receptor subfamily of transcription factors. It regulates transcription of genes involved in Insulin action, adipocyte differentiation, lipid metabolism and inflammation. It is implicated in numerous diseases including obesity, diabetes, atherosclerosis and cancer. It also activators include prostanoids, fatty acids, thiazolidinediones and N- (2-benzoylphenyl) tyrosine analogues. PPARG may modulate macrophage functions such as proinflammatory activities, and stimulate oxidized low-density lipoprotein (x-LDL) uptake.

NCBI Accession: NP_619725

Pathways: MAPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation