Tuberin antibody (AA 1611-1807)

Catalog No. ABIN7600203

This Mouse Monoclonal antibody specifically detects Tuberin in WB, IHC and FACS. It exhibits reactivity toward Human.

Quick Overview for Tuberin antibody (AA 1611-1807) (ABIN7600203)

Target: Tuberin (TSC2) (Tuberous Sclerosis 2 (TSC2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Tuberin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: 6I3

Product Details anti-Tuberin Antibody

Binding Specificity: AA 1611-1807

Purpose: Anti-Tuberin TSC2 Antibody (monoclonal, 6I3)

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: Human Tuberin shares 94%,90% amino acid (aa) sequence identity with mouse,rat Tuberin,respectively.

Characteristics: Anti-Tuberin TSC2 Antibody.(monoclonal, 6I3)-1. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Tuberin recombinant protein (Position: H1611-V1807). Human Tuberin shares 94% and 90% amino acid (aa) sequence identity with mouse and rat Tuberin, respectively.

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Tuberin

Target: Tuberin (TSC2) (Tuberous Sclerosis 2 (TSC2))

Alternative Name: TSC2

Background:

Background: Tuberous sclerosis 2 protein, also known as TSC2 or Tuberin is a protein that is in humans. The chromosomal location of this gene is 16p13.3. Mutations in this gene lead to tuberous sclerosis complex. Its gene product is believed to be a tumor suppressor and is able to stimulate specific GTPases. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. This gene involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling. It stimulates weakly the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5 in vitro.

Gene Full Name: TSC complex subunit 2

Molecular Weight: 280 kDa

Gene ID: 7249

UniProt: P49815

Pathways: RTK Signaling, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus