BRAF antibody (pThr401)

Catalog No. ABIN7603395

This Rabbit Monoclonal antibody specifically detects BRAF in WB, IF, ICC and IP. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for BRAF antibody (pThr401) (ABIN7603395)

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This BRAF antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Clone: IBE-2

Product Details anti-BRAF Antibody

Binding Specificity: pThr401

Purpose: Anti-Phospho-B Raf (T401) Rabbit Monoclonal Antibody

Characteristics: Anti-Phospho-B Raf Rabbit Monoclonal Antibody (ABIN7603395). Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human Phospho-B Raf (T401)

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000
ICC/IF 1:50-1:200
IP 1:30

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for BRAF

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Alternative Name: BRAF

Background:

Synonyms: Serine/threonine-protein kinase B-raf,2.7.11.1,Proto-oncogene B-Raf,p94,v-Raf murine sarcoma viral oncogene homolog B1,BRAF,BRAF1, RAFB1,

Tissue Specificity: Brain and testis.

Molecular Weight: 90-100 kDa

UniProt: P15056

Pathways: MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy