GZMB antibody

Catalog No. ABIN7605016

The Rabbit Monoclonal anti-GZMB antibody has been validated for IHC, IF, IP and ICC. It is suitable to detect GZMB in samples from Human.

Quick Overview for GZMB antibody (ABIN7605016)

Target: GZMB (Granzyme B (GZMB))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This GZMB antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: AAHC-7

Product Details anti-GZMB Antibody

Purpose: Anti-Granzyme B GZMB Monoclonal Antibody

Characteristics: Anti-Granzyme B GZMB Monoclonal Antibody (ABIN7605016). Tested in IHC, ICC/IF, IP applications. This antibody reacts with Human.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human Granzyme B This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.

Isotype: IgG

Application Details

Application Notes: IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: Lot specific

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for GZMB

Target: GZMB (Granzyme B (GZMB))

Alternative Name: GZMB

Background:

Synonyms: Leptin receptor,LEP-R,HuB219,OB receptor,OB-R,CD295,LEPR,DB, OBR,

Tissue Specificity: Isoform A is expressed in fetal liver and in hematopoietic tissues and choroid plexus. In adults highest expression in heart, liver, small intestine, prostate and ovary. Low level in lung and kidney. Isoform B is highly expressed in hypothalamus.

Molecular Weight: 23 kDa

UniProt: P10144

Pathways: Apoptosis, Caspase Cascade in Apoptosis