ISR-beta antibody (PE-Cy7)
-
- Target See all ISR-beta Antibodies
- ISR-beta (Insulin Receptor beta (ISR-beta))
-
Reactivity
- Human, Rat, Mouse
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This ISR-beta antibody is conjugated to PE-Cy7
-
Application
- Western Blotting (WB), Flow Cytometry (FACS)
- Cross-Reactivity
- Human, Rat
- Predicted Reactivity
- Mouse
- Purification
- Purified by Protein A.
- Immunogen
- KLH conjugated synthetic peptide derived from human IR beta
- Isotype
- IgG
- Top Product
- Discover our top product ISR-beta Primary Antibody
-
-
- Application Notes
-
FCM(1:100-500)
Optimal working dilution should be determined by the investigator. - Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
- Expiry Date
- 12 months
-
- Target
- ISR-beta (Insulin Receptor beta (ISR-beta))
- Alternative Name
- Insulin Receptor Beta (ISR-beta Products)
- Synonyms
- CD220 antibody, HHF5 antibody, 4932439J01Rik antibody, D630014A15Rik antibody, IR antibody, IR-A antibody, IR-B antibody, insulin receptor antibody, INSR antibody, Insr antibody
- Background
-
Synonyms: CD 220, CD220, CD220 antigen, HHF 5, HHF5, HIR B, INSR, INSR, Insulin receptor, Insulin receptor subunit beta, IR, INSR_HUMAN.
Background: The human insulin receptor is a heterotetrameric membrane glycoprotein consisting of disulfide linked subunits in a beta-alpha-alpha-beta configuration. The beta subunit (95 kDa) possesses a single transmembrane domain, whereas the alpha subunit (135 kDa) is completely extracellular. The insulin receptor exhibits receptor tyrosine kinase (RTK) activity. RTKs are single pass transmembrane receptors that possess intrinsic cytoplasmic enzymatic activity, catalyzing the transfer of the gamma phosphate of ATP to tyrosine residues in protein substrates. RTKs are essential components of signal transduction pathways that affect cell proliferation, differentiation, migration and metabolism.Included in this large protein family are the insulin receptor and the receptors for growth factors such as epidermal growth factor, fibroblast growth factor and vascular endothelial growth factor. Receptor activation occurs through ligand binding, which facilitates receptor dimerization and autophosphorylation of specific tyrosine residues in the cytoplasmic portion. The interaction of insulin with the alpha subunit of the insulin receptor activates the protein tyrosine kinase of the beta subunit, which then undergoes an autophosphorylation that increases its tyrosine kinase activity. Three adapter proteins, IRS1, IRS2 and Shc, become phosphorylated on tyrosine residues following insulin receptor activation. These three phosphorylated proteins then interact with SH2 domain containing signaling proteins.
- Molecular Weight
- 152kDa
- Gene ID
- 3643
- UniProt
- P06213
-