Myc Tag antibody (AA 408-437)

Catalog No. ABIN728893

Product Details anti-Myc Tag Antibody

Target: Myc Tag

Binding Specificity: AA 408-437

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Myc Tag antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Tag

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide corresponding to amino acids 408-437 of human Myc

Isotype: IgG

Application Details

Application Notes: WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for Myc Tag

Target: Myc Tag

Alternative Name: C-Myc Tag (Myc Tag Products)

Target Type: Tag

Background:

Synonyms: Avian myelocytomatosis viral oncogene homolog, bHLHe39, c Myc, c Myc Epitope Tag, c Myc Tag, Cellular myelocytomatosis oncogene, MGC105490, MRTL, Myc Epitope Tag, Myc protein, Myc proto oncogene protein, Myc proto-oncogene protein, Myc-related translation/localization regulatory factor, Myc2, myca, Myelocytomatosis oncogene a, Myelocytomatosis oncogene, Niard, Nird, Oncogene Myc, Protooncogene homologous to myelocytomatosis virus, RNCMYC, Transcription factor p64, Transcriptional regulator Myc-A, V-Myc avian myelocytomatosis viral oncogene homolog, v-myc myelocytomatosis viral oncogene homolog avian, zc-myc.

Background: Epitope tags are useful for the labeling and detection of proteins usingimmunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties. The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast,insect and mammalian cell systems.The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies also have shown that c-Myc is essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells, and which brought extensive attention in the development of new therapeutic approach for cancer treatment.

Gene ID: 4609