Bcl-2 antibody (Biotin)

Catalog No. ABIN7695682

The Rabbit Polyclonal anti-Bcl-2 antibody is suitable to detect Bcl-2 in samples from Human. It has been validated for WB.

Quick Overview for Bcl-2 antibody (Biotin) (ABIN7695682)

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Bcl-2 antibody is conjugated to Biotin

Application: Western Blotting (WB)

Product Details anti-Bcl-2 Antibody (Biotin)

Purpose: Biotin-Linked Polyclonal Antibody to B-Cell Leukemia/Lymphoma 2 (Bcl2)

Specificity: The antibody is a rabbit polyclonal antibody raised against Bcl2. It has been selected for its ability to recognize Bcl2 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500
Immunohistochemistry: 5-20 μg/mL,1:25-100
Immunocytochemistry: 5-20 μg/mL,1:25-100
Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for Bcl-2

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Alternative Name: B-Cell Leukemia/Lymphoma 2

Background: B-Cell CLL/Lymphoma 2

UniProt: P10415

Pathways: MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling