Caspase 4 antibody (Biotin)

Catalog No. ABIN7694942

This Rabbit Polyclonal antibody specifically detects Caspase 4 in WB, IHC and ICC. It exhibits reactivity toward Cow.

Quick Overview for Caspase 4 antibody (Biotin) (ABIN7694942)

Target: Caspase 4 (CASP4) (Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4))

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 4 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-Caspase 4 Antibody (Biotin)

Purpose: Biotin-Linked Polyclonal Antibody to Caspase 4 (CASP4)

Specificity: The antibody is a rabbit polyclonal antibody raised against CASP4. It has been selected for its ability to recognize CASP4 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500
Immunohistochemistry: 5-20 μg/mL,1:25-100
Immunocytochemistry: 5-20 μg/mL,1:25-100
Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for Caspase 4

Target: Caspase 4 (CASP4) (Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4))

Alternative Name: Caspase 4

Background: ICE(rel)II, ICH2, TX, Protease ICH-2, Cysteinyl Aspartate Specific Proteinases 4, Apoptosis-Related Cysteine Peptidase

UniProt: Q5E9C1

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity