CD4 antibody (Biotin)

Catalog No. ABIN7695776

This Rabbit Polyclonal antibody specifically detects CD4 in WB, IHC and ICC. It exhibits reactivity toward Human.

Quick Overview for CD4 antibody (Biotin) (ABIN7695776)

Target: CD4

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD4 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-CD4 Antibody (Biotin)

Purpose: Biotin-Linked Polyclonal Antibody to Cluster Of Differentiation 4 (CD4)

Specificity: The antibody is a rabbit polyclonal antibody raised against CD4. It has been selected for its ability to recognize CD4 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: PAB167Hu01Polyclonal Antibody to Cluster Of Differentiation 4 (CD4)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mLImmunocytochemistry in formalin fixed cells: 5-20 μg/mLImmunohistochemistry in formalin fixed frozen section: 5-20 μg/mLImmunohistochemistry in paraffin section: 5-20 μg/mLEnzyme-linked Immunosorbent Assay: 0.05-2 μg/mLOptimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for CD4

Target: CD4

Alternative Name: Cluster Of Differentiation 4

Background: CD4mut, p55, T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3

UniProt: P01730

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events