IGF1 antibody (Biotin)

Catalog No. ABIN7631941

This Rabbit Polyclonal antibody specifically detects IGF1 in WB, IHC and ICC. It exhibits reactivity toward Dog.

Quick Overview for IGF1 antibody (Biotin) (ABIN7631941)

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Reactivity: Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IGF1 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-IGF1 Antibody (Biotin)

Purpose: Biotin-Linked Polyclonal Antibody to Insulin Like Growth Factor 1 (IGF1)

Specificity: The antibody is a rabbit polyclonal antibody raised against IGF1. It has been selected for its ability to recognize IGF1 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: PAA050Ca01Polyclonal Antibody to Insulin Like Growth Factor 1 (IGF1)

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:50-400 Immunocytochemistry in formalin fixed cells: 1:50-500 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Immunohistochemistry in paraffin section: 1:10-100 Enzyme-linked Immunosorbent Assay: 1:100-1:5000 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for IGF1

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Alternative Name: Insulin Like Growth Factor 1

Background: IGFI, IGF1A, IBP1, MGF, Somatomedin C, Mechano Growth Gactor

UniProt: P33712

Pathways: RTK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation