PAI1 antibody (Biotin)

Catalog No. ABIN7632875

The Mouse Monoclonal anti-PAI1 antibody has been validated for WB, IHC and ICC. It is suitable to detect PAI1 in samples from Human.

Quick Overview for PAI1 antibody (Biotin) (ABIN7632875)

Target: PAI1 (SERPINE1) (Plasminogen Activator Inhibitor 1 (SERPINE1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PAI1 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-PAI1 Antibody (Biotin)

Purpose: Biotin-Linked Monoclonal Antibody to Plasminogen Activator Inhibitor 1 (PAI1)

Specificity: The antibody is a mouse monoclonal antibody raised against PAI1. It has been selected for its ability to recognize PAI1 in immunohistochemical staining and western blotting.

Purification: Protein A + Protein G affinity chromatography

Immunogen: MAA532Hu24Monoclonal Antibody to Plasminogen Activator Inhibitor 1 (PAI1)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for PAI1

Target: PAI1 (SERPINE1) (Plasminogen Activator Inhibitor 1 (SERPINE1))

Alternative Name: Plasminogen Activator Inhibitor 1

Background: SERPINE1, PLANH1, Serpin Peptidase Inhibitor Clade E Member 1, Nexin,Plasminogen Activator Inhibitor Type 1 Serpin E1, Endothelial plasminogen activator inhibitor

UniProt: P05121

Pathways: p53 Signaling, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy, Smooth Muscle Cell Migration