CD45RA antibody (Biotin)

Catalog No. ABIN7651579

This Mouse Monoclonal antibody specifically detects CD45RA in FACS, IHC, WB and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for CD45RA antibody (Biotin) (ABIN7651579)

Target: CD45RA

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD45RA antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: K4B5

Product Details anti-CD45RA Antibody (Biotin)

Purpose: CD45RA (Leukocyte Marker)(K4B5), Biotin conjugate

Characteristics: Recognizes a protein of 205 kDa-220 kDa, identified as CD45RA. CD45RA is isoforms of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50 % of peripheral CD4 T-cells, 50 % of peripheral CD8 T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This MAb is useful in study on the subpopulation of CD4 or CD8 T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO ve) from B cell lymphomas (CD45RA ve). Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Stimulated human leukocytes

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: Daudi, Raji, IM9 and human lymphocytes. Human spleen, lymph nodes and tonsils.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for CD45RA

Target: CD45RA

Alternative Name: CD45RA

Background:

Synonyms: B220, CD45R, GP180, Leukocyte common antigen (LCA), Loc, Ly-5, Lyt-4, Protein tyrosine phosphatase receptor type C (PTPRC), Receptor-type tyrosine-protein phosphatase C, T200 glycoprotein

Gene Symbol: PTPRC

Tissue Expression: B-cells

Molecular Weight: 205-220 kDa

Gene ID: 5788

UniProt: P08575