GZMB antibody (AA 73-187) (CF®488A)

Catalog No. ABIN7654681

This Mouse Monoclonal antibody specifically detects GZMB in IHC and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for GZMB antibody (AA 73-187) (CF®488A) (ABIN7654681)

Target: GZMB (Granzyme B (GZMB))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GZMB antibody is conjugated to CF®488A

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: GZMB-3056

Product Details anti-GZMB Antibody (CF®488A)

Binding Specificity: AA 73-187

Purpose: Granzyme B (NK/T-Cell Lymphoma Marker)(GZMB/3056), CF488A conjugate

Characteristics: Granzyme B is a member of the granule serine protease family stored specifically in NK cells or cytotoxic T cells. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. Granzyme B is crucial for the rapid induction of target cell apoptosis by CTLs in the cell-mediated immune response. Granzyme B is useful as a marker in the identification of NK/T-cell lymphomas. High percentages of cytotoxic T-cells have been shown to be an unfavorable prognostic indicator in Hodgkin's Disease. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment of human GZMB protein (around aa 73-187)

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: A431 cells. Human tonsil, spleen or Hodgkin Lymphoma tissue (IHC).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for GZMB

Target: GZMB (Granzyme B (GZMB))

Alternative Name: Granzyme B

Background:

Synonyms: Cathepsin G-like 1, CCPI, CGL1, CSPB, CTLA-1, CTSGL1, Cytotoxic serine protease B, Cytotoxic T lymphocyte associated serine esterase 1, Cytotoxic T-lymphocyte proteinase 2, Fragmentin-2, GRB, Human lymphocyte protein (Hlp), Lymphocyte protease, SECT, T-cell serine protease 1-3E

Gene Symbol: GZMB

Tissue Expression: NK cells|T-cells

Molecular Weight: 29-32 kDa

Gene ID: 3002

UniProt: P10144

Pathways: Apoptosis, Caspase Cascade in Apoptosis