PCNA antibody (CF®740)

Catalog No. ABIN7659196

This Mouse Monoclonal antibody specifically detects PCNA in IHC, FACS and IHC (fp). It exhibits reactivity toward Human, Mouse, Rat, Chicken, Monkey, Pig, Saccharomyces cerevisiae, Zebrafish (Danio rerio), Drosophila melanogaster and Schizosaccharomyces pombe.

Quick Overview for PCNA antibody (CF®740) (ABIN7659196)

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reactivity: Human, Mouse, Rat, Chicken, Monkey, Pig, Saccharomyces cerevisiae, Zebrafish (Danio rerio), Drosophila melanogaster, Schizosaccharomyces pombe

Host: Mouse

Clonality: Monoclonal

Conjugate: This PCNA antibody is conjugated to CF®740

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: PC10

Product Details anti-PCNA Antibody (CF®740)

Purpose: PCNA(PC10), CF740 conjugate

Characteristics: Recognizes a non-histone protein of 36 kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This MAb is excellent for multiple applications. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Rat PCNA/Protein A fusion protein

Isotype: IgG2a, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Immunohistology formalin-fixed 0.25-0.5 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Western blotting 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: Tonsil or reactive lymph node

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Alternative Name: PCNA

Background:

Synonyms: Cyclin, DNA polymerase delta auxiliary protein, Mutagen-sensitive 209 protein, PCNAR, Polymerase delta accessory protein

Gene Symbol: PCNA

Tissue Expression: Proliferating cells

Molecular Weight: 36 kDa

Gene ID: 5111

UniProt: P12004

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy