IL12 antibody

Catalog No. ABIN7670659

This anti-IL12 antibody is a Rabbit Polyclonal antibody detecting IL12 in ELISA. Suitable for Rat.

Quick Overview for IL12 antibody (ABIN7670659)

Target: IL12 (Interleukin 12 (IL12))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL12 antibody is un-conjugated

Application: ELISA

Product Details anti-IL12 Antibody

Purpose: IL-12 p70 Polyclonal Antibody(Capture/Detector)

Purification: Antigen Affinity Purification

Immunogen: Recombinant Rat IL-12 p70 protein expressed by Mammalian

Isotype: IgG

Application Details

Application Notes: ELISA Capture 2-8 μg/mL,ELISA Detector 0.1-0.4 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Sterile PBS , pH 7.4

Preservative: Without preservative

Handling Advice: Avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C Valid for 1 month or -20°C Valid for 12 months

Expiry Date: 12 months

Target Details for IL12

Target: IL12 (Interleukin 12 (IL12))

Alternative Name: IL-12 p70

Background: IL-12A,IL12a,InterLeukin-12 subunit aLpha,Cytotoxic Lymphocyte maturation factor 35 kDa subunit (CLMF p35),IL-12 subunit p35,IL12b,IL12B,InterLeukin-12 subunit beta,Cytotoxic Lymphocyte maturation factor 40 kDa subunit,IL-12 subunit p40,Heterodimerizes with Il12B to form the Il-12 cytokine or with EBI3/Il27B to form the Il-35 cytokine. Il-12 is primarily produced by professional antigen-presenting cells (APCs) such as B-cells and dendritic cells (DCs) as well as macrophages and granulocytes and regulates T-cell and natural killer-cell responses,induces the production of interferon-gamma (IFN-gamma),favors the differentiation of T-helper 1 (Th1) cells and is an important link between innate resistance and adaptive immunity. Mechanistically,exerts its biological effects through a receptor composed of Il12R1 and Il12R2 subunits. Binding to the receptor results in the rapid tyrosine phosphorylation of a number of cellular substrates including the JAK family kinases TYK2 and JAK2. In turn,recruited STAT4 gets phosphorylated and translocates to the nucleus where it regulates cytokine/growth factor responsive genes.As part of Il-35,plays essential roles in maintaining the immune homeostasis of the liver microenvironment and functions also as an immune-suppressive cytokine.Mediates biological events through unconventional receptors composed of Il12RB2 and gp130/Il6ST heterodimers or homodimers. Signaling requires the transcription factors STAT1 and STAT4,which form a unique heterodimer that binds to distinct DNA sites.

UniProt: Q9R103, Q9R278

Pathways: JAK-STAT Signaling, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome