CYP3A4 antibody (AA 18-120)

Catalog No. ABIN733793

Product Details anti-CYP3A4 Antibody

Target: CYP3A4 (Cytochrome P450, Family 3, Subfamily A, Polypeptide 4 (CYP3A4))

Binding Specificity: AA 18-120

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CYP3A4 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Will detect CYP 3A4, 3A7, 3A5, and 3A9

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Pig,Rabbit,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CYP3A4

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-CYP3A4 antibody (ABIN733793)

Ito, Otsuki, Zhang, Konno, Kurahashi, Takahashi, Yamato, Matsuoka, Yamada, Miyata, Fujii: "Ascorbic acid reverses the prolonged anesthetic action of pentobarbital in Akr1a-knockout mice." in: Life sciences, Vol. 95, Issue 1, pp. 1-8, (2014) (PubMed).

Target Details for CYP3A4

Target: CYP3A4 (Cytochrome P450, Family 3, Subfamily A, Polypeptide 4 (CYP3A4))

Alternative Name: CYP3A4 (CYP3A4 Products)

Synonyms: CP33 antibody, CP34 antibody, CYP3A antibody, CYP3A3 antibody, CYPIIIA3 antibody, CYPIIIA4 antibody, HLP antibody, NF-25 antibody, P450C3 antibody, P450PCN1 antibody, MGC108372 antibody, CYP3A80 antibody, CYP3A4 antibody, CYP3A21 antibody, CYPIIIA21 antibody, CYP3A12 antibody, cytochrome P450 family 3 subfamily A member 4 antibody, cytochrome P450 family 3 subfamily A member 43 antibody, cytochrome P450 3A4 antibody, cytochrome P450, subfamily IIIA, polypeptide 4 antibody, cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4 antibody, cytochrome P450, family 3, subfamily A, polypeptide 4 antibody, CYP3A4 antibody, CYP3A43 antibody, PTRG_01782 antibody, PTRG_06060 antibody, cyp3a4 antibody

Background:

Synonyms: Cytochrome p450 3A4, CP33, CP34, CYP3A, CYP3A3, CYP3A4, CYPIIIA4, CYPIIIA3, Cytochrome P450 family 3 subfamily A polypeptide 4, Cytochrome P450 subfamily IIIA polypeptide 4, Glucocorticoid inducible P450, HLP, MGC126680, NF 25, NF25, P450 III steroid inducible, P450 PCN1, P450C3, P450PCN1, Quinine 3 monooxygenase, Taurochenodeoxycholate 6 alpha hydroxylase, CP3A4_HUMAN, 1,8-cineole 2-exo-monooxygenase, Albendazole monooxygenase, Albendazole sulfoxidase, Cytochrome P450 3A3, Cytochrome P450 HLp, Cytochrome P450 NF-25, Cytochrome P450-PCN1, Nedipine oxidase, Quinine 3-monooxygenase, Taurochenodeoxycholate 6-alpha-hydroxylase, CYP3A4, CYP3A7, CYP3A5, CYP3A9

Background: Cytochromes P450 are a group of heme thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8 oxidation, omeprazole sulphoxidation, midazolam 1 hydroxylation and midazolam 4 hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. The enzyme also hydroxylates etoposide.

Molecular Weight: 46kDa

Gene ID: 1576

Pathways: Steroid Hormone Biosynthesis