ALIX antibody

Catalog No. ABIN7673519

This Mouse Monoclonal antibody specifically detects ALIX in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ALIX antibody (ABIN7673519)

Target: ALIX (PDCD6IP) (Programmed Cell Death 6 Interacting Protein (PDCD6IP))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ALIX antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 8H4

Product Details anti-ALIX Antibody

Purpose: PDCD6IP antibody

Purification: protein A+G purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: programmed cell death 6 interacting protein

Isotype: IgG1

Application Details

Application Notes: WB: 1:500 - 1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for ALIX

Target: ALIX (PDCD6IP) (Programmed Cell Death 6 Interacting Protein (PDCD6IP))

Alternative Name: PDCD6IP

Background:

Synonyms: Programmed cell death 6-interacting protein (PDCD6-interacting protein)|ALG-2-interacting protein 1|ALG-2-interacting protein X|Hp95|PDCD6IP|AIP1|ALIX|KIAA1375

Background: This gene encodes a protein that functions within the ESCRT pathway in the abscission stage of cytokinesis, in intralumenal endosomal vesicle formation, and in enveloped virus budding. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. Related pseudogenes have been identified on chromosome 15.

Molecular Weight: 100 kDa

Gene ID: 10015

UniProt: Q8WUM4

Pathways: p53 Signaling, EGFR Signaling Pathway, Sensory Perception of Sound, Cellular Response to Molecule of Bacterial Origin, Tube Formation