Recombinant Bcl-2 antibody

Catalog No. ABIN7797721

The Rabbit Monoclonal anti-Bcl-2 antibody (Clone 25GB3660) (ABIN7797721) specifically detects Bcl-2 in WB and FACS. The antibody is reactive with Human samples.

Quick Overview for Recombinant Bcl-2 antibody (ABIN7797721)

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Bcl-2 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Grade: KD Validated

Clone: 25GB3660

Product Details anti-Bcl-2 Antibody

Expression System: HEK-293 Cells

Purpose: KD-Validated Bcl2 Recombinant Rabbit mAb

Cross-Reactivity: Mouse

Purification: Affinity Purified

Immunogen: Recombinant protein of human Bcl-2

Isotype: IgG

Application Details

Application Notes: Western Blotting (WB): 1:1,000-1:5,000, Flow Cytometry (FCM): 1:200-1:2,000

Comment:

We assess the antibodies’ specificity and selectivity using knockdown techniques. Our validation approach is straightforward: if an antibody identifies a signal in wild-type (WT) cells, but this signal is absent or noticeably reduced in gene-silenced (KD) cells, we consider the antibody to be highly specific.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS (pH 7.4) containing 50% glycerol and 0.02% sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C for one year.

Expiry Date: 12 months

Target Details for Bcl-2

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Alternative Name: Bcl2

Molecular Weight: 26 kDa

Gene ID: 596

UniProt: P10415

Pathways: MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling