Src antibody (AA 2-100) (Cy5.5)

Catalog No. ABIN738176

Product Details anti-Src Antibody (Cy5.5)

Target: Src (Proto-oncogene tyrosine-protein kinase Src (Src))

Binding Specificity: AA 2-100

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Src antibody is conjugated to Cy5.5

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human SRC

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Src

Target: Src (Proto-oncogene tyrosine-protein kinase Src (Src))

Alternative Name: SRC (Src Products)

Synonyms: AW259666 antibody, pp60c-src antibody, C-src1 antibody, CG7524 antibody, D-Src64B antibody, D-src antibody, DSRC64 antibody, DSrc antibody, DSrc64 antibody, DSrc64B antibody, Dm SRC1 antibody, DmHD-358 antibody, Dmel\CG7524 antibody, Dsrc antibody, Dsrc64 antibody, Dsrc64B antibody, HD-358 antibody, MRE5 antibody, SRC 64B antibody, Src antibody, Src1 antibody, Src64 antibody, c-src antibody, dSrc antibody, dsrc antibody, src antibody, src1 antibody, src64 antibody, src64B antibody, src64b antibody, ASV antibody, SRC1 antibody, c-SRC antibody, p60-Src antibody, c-Src antibody, PP60C-SCR antibody, SDR antibody, SRC proto-oncogene, non-receptor tyrosine kinase antibody, uncharacterized LOC5574802 antibody, proto-oncogene tyrosine-protein kinase src antibody, Rous sarcoma oncogene antibody, Src oncogene at 64B antibody, SRC antibody, LOC5574802 antibody, CpipJ_CPIJ010659 antibody, Src antibody, Src64B antibody

Target Type: Viral Protein

Background:

Synonyms: ASV, Avian sarcoma virus, c SRC, CDNA FLJ14219 fis clone NT2RP3003800 highly similar to Rattus norvegicus tyrosine protein kinase pp60 c src mRNA, cSrc, EC 2.7.10.2, Neuronal CSRC tyrosine specic protein kinase, Neuronal SRC, Oncogene SRC, OTTHUMP00000030931, OTTHUMP00000174476, OTTHUMP00000174477, p60 Src, p60-Src, p60Src, pp60c src, pp60c-src, pp60csrc, Proto oncogene tyrosine protein kinase Src, Proto-oncogene c-Src, Proto-oncogene tyrosine-protein kinase Src, Protooncogene SRC, protooncogene SRC Rous sarcoma, Src, SRC Oncogene, SRC_MOUSE, SRC1, Tyrosine kinase pp60c src, Tyrosine protein kinase SRC 1, Tyrosine protein kinase SRC1, v src avian sarcoma Schmidt Ruppin A2 viral oncogene homolog, V src sarcoma Schmidt Ruppin A 2 viral oncogene homolog avian, v src sarcoma Schmidt Ruppin A 2 viral oncogene homolog avian, vsrc avian sarcoma Schmidt Ruppin A2 viral oncogene homolog, SRC_HUMAN.

Background: c-Src tyrosine kinase plays a critical role in signal transduction downstream of growth factor receptors, integrins and G protein-coupled receptors. We used stable isotope labeling with amino acids in cell culture (SILAC) approach to identify additional substrates of c-Src tyrosine kinase in human embryonic kidney 293T cells. We have identified 10 known substrates and interactors of c-Src and Src family kinases along with 26 novel substrates. We have experimentally validated 4 of the novel proteins (NICE-4, RNA binding motif 10, FUSE-binding protein 1 and TRK-fused gene) as direct substrates of c-Src using in vitro kinase assays and cotransfection experiments. Significantly, using a c-Src specific inhibitor, we were also able to implicate 3 novel substrates (RNA binding motif 10, EWS1 and Bcl-2 associated transcription factor) in PDGF signaling. Finally, to identify the exact tyrosine residues that are phosphorylated by c-Src on the novel c-Src substrates, we designed custom peptide microarrays containing all possible tyrosine-containing peptides (312 unique peptides) and their mutant counterparts containing a Tyr --> Phe substitution from 14 of the identified substrates. Using this platform, we identified 34 peptides that are phosphorylated by c-Src. We have demonstrated that SILAC-based quantitative proteomics approach is suitable for identification of substrates of nonreceptor tyrosine kinases and can be coupled with peptide microarrays for high-throughput identification of substrate phosphopeptides.

Gene ID: 6714

UniProt: P12931

Pathways: JAK-STAT Signaling, Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Cellular Response to Molecule of Bacterial Origin, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling, Platelet-derived growth Factor Receptor Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling