KIT antibody (C-Term)

Catalog No. ABIN7825521

This Rabbit Polyclonal antibody specifically detects KIT in WB and IHC. It exhibits reactivity toward Human.

Quick Overview for KIT antibody (C-Term) (ABIN7825521)

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KIT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-KIT Antibody

Binding Specificity: C-Term

Purpose: Anti-c-Kit/Kit Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-c-Kit/Kit Antibody. Tested in IHC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse c-Kit/Kit, which shares 88.6% and 94.3% amino acid (aa) sequence identity with human and rat c-Kit/Kit, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for KIT

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Alternative Name: Kit

Background:

Background: SCFR (Mast/stem cell growth factor receptor), also known as KIT ONCOGENE or CD117, is a protein that in humans is encoded by the KIT gene. KIT was first described as the cellular homolog of the feline sarcoma viral oncogene v-kit. The KIT gene is mapped on 4q12. Kit was expressed on the surface of germ cells up to the pachytene stage. Signaling from the KIT receptor tyrosine kinase is essential for primordial germ cell growth both in vivo and in vitro. Determination of the KIT effectors acting in primordial germ cells has been hampered by the lack of effective methods to manipulate easily gene expression in these cells.

Gene Full Name: Kit proto-oncogene receptor tyrosine kinase

Molecular Weight: 145 kDa

Gene ID: 3815

UniProt: P05532

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity