Recombinant SDHB antibody
Quick Overview for Recombinant SDHB antibody (ABIN7826143)
Target
See all SDHB AntibodiesAntibody Type
Reactivity
Host
Clonality
Conjugate
Application
Clone
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Purpose
- SDHB antibody (HMV320)
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Immunogen
- Recombinant human SDHB fragment
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Isotype
- IgG
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Application Notes
- IHC 1:100-1:200
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Comment
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Positive Control: Appendix: A strong SDHB staining should be seen in epithelial cells and a weak to moderate staining should occur in lymphocytic cells.
Product Description/Background: SDHB antibody validated for immunohistochemistry on 76 different Normal Tissues
Negative Control: SDHB staining should be absent in all cells of a tumor with known SDH deficiency.
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Protocol
- Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.
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Restrictions
- For Research Use only
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Format
- Liquid
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
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Expiry Date
- 24 months
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- SDHB (Succinate Dehydrogenase Complex, Subunit B, Iron Sulfur (Ip) (SDHB))
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Alternative Name
- SDHB
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Background
- Gene Alias: succinate dehydrogenase complex iron sulfur subunit B,CWS2,IP,PGL4,SDH,SDH1,SDH2,SDHIP
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UniProt
- P21912
Target
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