GFP antibody

Catalog No. ABIN7833281

The Rabbit Polyclonal anti-GFP antibody (ABIN7833281) specifically detects GFP in WB. The antibody is reactive with Aequorea victoria samples.

Quick Overview for GFP antibody (ABIN7833281)

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GFP antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GFP Antibody

Purpose: Anti-GFP | Green fluorescent protein (VENUS)

Cross-Reactivity (Details): cell lysate overexoressing Venus protein fusion
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Purification: Immunogen affinity purified serum

Immunogen: Full length

Application Details

Application Notes: 1 : 1000 (WB)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL, of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Lyophilized antibody can be stored at -20°C for up to 3 years. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Expiry Date: 36 months

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP

Background: Green fluorescent protein (Venus) from Aequorea victoria (Jellyfish), can be mutated to emit at different wavelengths such as blue for BFP (when Tyr-66 is replaced by His), cyan for CFP (when Tyr-66 is replaced by Trp), and yellow for YFP (when THR-203 is replaced by Tyr). It is an energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer.

Molecular Weight: 26 kDa

UniProt: P42212