GFP antibody

Catalog No. ABIN7833282

This Rabbit Polyclonal antibody specifically detects GFP in WB, IP and ChIP. It exhibits reactivity toward Aequorea victoria, Chlamydomonas reinhardtii and Nicotiana tabacum.

Quick Overview for GFP antibody (ABIN7833282)

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria, Chlamydomonas reinhardtii, Nicotiana tabacum

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GFP antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)

Product Details anti-GFP Antibody

Purpose: Anti-GFP | Green Fluorescence Protein

Cross-Reactivity (Details): Recombinant GFP overexpressed in E.coli from Arabidopsis thaliana, Chlamydomonas reinhardii, Nicotiana tabacum

Predicted Reactivity: mClover3

Purification: Immunogen affinity purified serum

Immunogen: Recombinant GFP protein derived from Aequorea victoria

Application Details

Application Notes: 1: 5000 - 1 : 10 000 (WB)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP

Background: GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa.