GFP antibody

Catalog No. ABIN7833283

The Rat Monoclonal anti-GFP antibody is suitable to detect GFP in samples from Aequorea victoria. It has been validated for WB, ELISA, IF, IP and ChIP.

Quick Overview for GFP antibody (ABIN7833283)

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Rat

Clonality: Monoclonal

Conjugate: This GFP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)

Clone: 1A5 (rat monoclonal

Product Details anti-GFP Antibody

Purpose: Anti-GFP | Green Fluorescence Protein, clone 1A5 (rat monoclonal)

Cross-Reactivity (Details): Native GFP, Recombinant GFP (E. coli), all variants of GFP, including EGFP

Purification: Protein A purified

Sterility: Sterile filtered

Immunogen: Recombinant GFP protein from Aequorea victoria

Isotype: IgG1

Application Details

Application Notes: 1 : 5000-1 : 25 000 (ELISA), 1 : 500 (IF), 1 : 2000-1 : 10 000 (WB)

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Immunoglobulin Protein A purified in PBS, 50 % glycerol, filter sterilized.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store at -20°C, Avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP

Background:

Background: GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa.

Additional information: Antibody detects GFP and EGFP

UniProt: P42212