FLT3 antibody

Catalog No. ABIN7835738

The Mouse Monoclonal anti-FLT3 antibody (Clone BV10-A4H2) (ABIN7835738) specifically detects FLT3 in FACS and IF. The antibody is reactive with Human samples.

Quick Overview for FLT3 antibody (ABIN7835738)

Target: FLT3 (Fms-Related tyrosine Kinase 3 (FLT3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FLT3 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: BV10-A4H2

Product Details anti-FLT3 Antibody

Purpose: Mouse monoclonal antibody to CD135

Specificity: Recognizes human CD135

Purification: The antibody was purified by affinity chromatography.

Immunogen: BV-173 pro-B cell line

Application Details

Application Notes: IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Target Details for FLT3

Target: FLT3 (Fms-Related tyrosine Kinase 3 (FLT3))

Alternative Name: FLT3

Background: CD135, FLK2, STK1, Receptor-type tyrosine-protein kinase FLT3, FL cytokine receptor, Fetal liver kinase-2, FLK-2, Fms-like tyrosine kinase 3, FLT-3, Stem cell tyrosine kinase 1, STK-1, CD135

Gene ID: 2322

UniProt: P36888

Pathways: RTK Signaling