Recombinant Choline Acetyltransferase antibody (C-Term)

Catalog No. ABIN7865483

The Mouse Monoclonal anti-Choline Acetyltransferase antibody is suitable to detect Choline Acetyltransferase in samples from Human. It has been validated for IHC.

Quick Overview for Recombinant Choline Acetyltransferase antibody (C-Term) (ABIN7865483)

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Choline Acetyltransferase antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: R38B12

Product Details anti-Choline Acetyltransferase Antibody

Binding Specificity: C-Term

Expression System: CHO Cells

Purpose: Recombinant Choline Acetyltransferase (CHAT) (Marker of Cholinergic Cells) Antibody

Specificity: Choline acetyltransferase (ChAT) is a 68 kD enzyme which catalyses the synthesis of acetylcholine (Ach) from choline and acetyl coenzyme A. The human ChAT gene encodes two proteins, the 68 kD ChAT enzyme and a 27 kD protein immunologically related and coexpressed with ChAT in cholinergic neurons of the central nervous system. The smaller proteins may play a role in the regulation of ACh synthesis. ChAT is expressed in cholinergic neurons, the majority of the neurons in the nucleus basalis of Meynert, large neurones in the striatum (putamen and caudate nuclei), the majority of neurones in the pedunculo-pontine, hypoglossal, dorsal nucleus of vagus and subgroups of neurones in Roller,Ä111s and the medial olivary accessory nuclei. Prominent staining is observed in ribonucleoprotein, distributed at the periphery of large neurons of the nucleus basalis of Meynert, the motor neurons in the hypoglossal and ambiguus nuclei. NCL-ChAT does not label axons in the insular cortex of the internal capsule non-cholinergic structures, endothelial cells or microglia.

Purification: 200ug/ml of Ab produced in CHO cell mammalian-based expression system.

Immunogen: Prokaryotic recombinant protein corresponding to the C-terminal region of the human ChAT molecule.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for Choline Acetyltransferase

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Alternative Name: Choline Acetyltransferase

Background:

CMS1A | CMS1A2 CMS6

Human appendix, colon, stomach or brain.

Molecular Weight: 82kDa

Gene ID: 1103, 410969

UniProt: P28329

Pathways: Skeletal Muscle Fiber Development