Recombinant Estrogen Receptor alpha antibody (AA 495-595)

Catalog No. ABIN7864310

This Rabbit Monoclonal antibody specifically detects Estrogen Receptor alpha in ELISA. It exhibits reactivity toward Human.

Quick Overview for Recombinant Estrogen Receptor alpha antibody (AA 495-595) (ABIN7864310)

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Estrogen Receptor alpha antibody is un-conjugated

Application: ELISA

Clone: ESR1-8206R

Product Details anti-Estrogen Receptor alpha Antibody

Binding Specificity: AA 495-595

Purpose: Recombinant Estrogen Receptor, alpha (Marker of Estrogen Dependence) Antibody

Specificity: Estrogen receptor (ER) belongs to the steroid receptor superfamily of nuclear receptors. It is a protein with 553 amino acids. The receptor molecule has three domains, i.e. the central DNA-binding domain, the hormone-binding domain at the C-terminal, and the transcription-activating domain at the N-terminal. ER mediates regulatory functions of female sex steroids, mainly 17 (E2), on growth, differentiation and function in several target tissues, including female and male reproductive tract, mammary gland, and skeletal and cardiovascular systems. Studies have shown ER é 79oΩ is present in the nuclei of epithelial cells in normal breast and endometrial tissues, as well as a subset of breast carcinomas. Studies with immunohistochemical assay show that positive steroid hormone status has predicted favorable overall, survival, independently of hormonal treatment. Secondly, ER é 79oΩ can be used as a tumor marker, preferentially in combination with an antibody to progesterone receptor, e.g., in the classification of adenocarcinomas.

Purification: 200ug/ml of Ab purified by Protein A/G.

Immunogen: Recombinant fragment (around aa495-595) of human ER alpha protein (exact sequence is proprietary)

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for Estrogen Receptor alpha

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Alternative Name: Estrogen Receptor, alpha

Background:

Estrogen Receptor alpha delta 4*5,6,7*/654 isoform, Estrogen Receptor alpha delta 4 +49 isoform, Nuclear receptor subfamily 3 group A member 1

MCF-7 cells. Human breast or endometrial carcinoma.

Molecular Weight: ~67kDa

Gene ID: 2099, 208124

UniProt: P03372

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly