Recombinant Estrogen Receptor alpha antibody (C-Term)

Catalog No. ABIN7858031

This Mouse Monoclonal antibody specifically detects Estrogen Receptor alpha in WB and IHC. It exhibits reactivity toward Human.

Quick Overview for Recombinant Estrogen Receptor alpha antibody (C-Term) (ABIN7858031)

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Estrogen Receptor alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: RESR1-9525

Product Details anti-Estrogen Receptor alpha Antibody

Binding Specificity: C-Term

Purpose: Recombinant Estrogen Receptor, alpha (Marker of Estrogen Dependence) Antibody

Specificity: Recognizes a protein of 67 kDa, which is identified as estrogen receptor (ER) alpha. The ER gene consists of more than 140kb of genomic DNA divided into 8 exons, being translated into a protein with six functionally discrete domains, labeled A through F. This antibody strongly stains the nucleus of epithelial cells in breast carcinomas.The ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to antiestrogen (e.g. tamoxifen) therapy.

Purification: 200ug/ml of Ab purified by Protein A/G.

Immunogen: Recombinant fragment corresponding to C-terminus of human ESR1 protein (exact sequence is proprietary)

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for Estrogen Receptor alpha

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Alternative Name: Estrogen Receptor, alpha

Background:

Estrogen Receptor alpha delta 4*5,6,7*/654 isoform, Estrogen Receptor alpha delta 4 +49 isoform, Nuclear receptor subfamily 3 group A member 1

HeLa, MCF7 cells. Human breast or endometrial carcinoma.

Molecular Weight: ~67kDa

Gene ID: 2099, 208124

UniProt: P03372

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly