Bcl-2 antibody (AA 1-200)

Catalog No. ABIN7858364

This Mouse Monoclonal antibody specifically detects Bcl-2 in IHC. It exhibits reactivity toward Human.

Quick Overview for Bcl-2 antibody (AA 1-200) (ABIN7858364)

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Bcl-2 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: BCL2-6915

Product Details anti-Bcl-2 Antibody

Binding Specificity: AA 1-200

Purpose: Bcl-2 (Apoptosis & Follicular Lymphoma Marker) Antibody

Specificity: This antibody recognizes a protein of 25-26 kDa, identified as the bcl-279 79oΩ 79oΩlpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-279 79oΩ 79oΩlpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-279 79oΩ 79oΩlpha protein, whereas the normal or hyperplastic germinal centers are negative.Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa1-200) of human BCL2 protein (exact sequence is proprietary)

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for Bcl-2

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Alternative Name: Bcl-2

Background:

Apoptosis regulator Bcl-2, B-cell CLL/lymphoma-2

HL-60|Jurkat|K562|or HeLa cells. Tonsil or follicular lymphomas.

Molecular Weight: 25-26kDa

Gene ID: 596, 150749

Pathways: MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling