CD11b antibody (AA 900-1100)

Catalog No. ABIN7860652

The Mouse Monoclonal anti-CD11b antibody (Clone ITGAM-4741) (ABIN7860652) specifically detects CD11b in IHC and WB. The antibody is reactive with Human, Mouse and Guinea Pig samples.

Quick Overview for CD11b antibody (AA 900-1100) (ABIN7860652)

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reactivity: Human, Mouse, Guinea Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD11b antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Clone: ITGAM-4741

Product Details anti-CD11b Antibody

Binding Specificity: AA 900-1100

Purpose: CD11b / MAC-1 (Microglial Marker) Antibody

Specificity: CD11b is a cell adhesion molecule that acts as a receptor for cell surface ligands such as intracellular adhesion molecules (ICAMs) or soluble ligands. Integrins are heterodimeric proteins that contain an a chain and b chain. Integrin 69 79oΩM combines with the Integrin 79oΩ 79oΩ2 to form a leukocyte-specific integrin referred to as macrophage receptor 1 (Mac-1), or inactivated-C3b (iC3b) receptor 3 (CR3). Integrin 69 79oΩM/ 79oΩ 79oΩ2 is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. The protein CD11b has been implicated in the various adhesion-related interactions of cells such as monocytes, macrophages, natural killer (NK) cells, and granulocytes. It is part of a heterodimer that consists of CD11b and 79oΩCD18. It also modulates the uptake of complement-coated particles within the cell. It is commonly used as a microglial marker in tissues derived from the nervous system.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa900-1100) of human ITGAM protein (exact sequence is proprietary)

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for CD11b

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Alternative Name: CD11b

Background:

CD18; CD49d; Cell surface glycoprotein MAC-1 subunit alpha; Complement Component Receptor 3 Alpha; CR3 Alpha Chain (CR3A); Integrin alpha-M; Integrin beta 2 alpha subunit; Leukocyte adhesion receptor MO1; Ly-40; MAC1A; Macrophage antigen alpha polypeptide; MO1A; Neutrophil adherence receptor alpha M subunit

Monocytes & granulocytes. Lymph nodes|spleen and tonsil (IHC). Lung

Molecular Weight: 95kDa & 170kDa

Gene ID: 3684, 172631

UniProt: P11215

Pathways: Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation