Recombinant CDK4 antibody (N-Term)

Catalog No. ABIN7865043

The Rabbit Monoclonal anti-CDK4 antibody is suitable to detect CDK4 in samples from Human. It has been validated for WB and IHC.

Quick Overview for Recombinant CDK4 antibody (N-Term) (ABIN7865043)

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CDK4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: CDK4-8210R

Product Details anti-CDK4 Antibody

Binding Specificity: N-Term

Purpose: Recombinant CDK4 Antibody

Specificity: Cyclin-dependent kinase-4 (CDK4) is a protein-serine kinase involved in the cell cycle. It is essential for the G1- to S-phase transition during the cell cycle and its expression is primarily controlled at the transcriptional level (PMID:17253961). CCND1- CDK4 axis is not only critical in glial tumor cells but also in stromal-derived cells in the surrounding tumor microenvironment that are vital to sustain tumor outgrowth (PMID:21844184). CDK4 is highly expressed in highly differentiated and dedifferentiated liposarcomas, but rarely expressed in other benign liposarcomas and other sarcomas. CDK4 and MDM2 combined to differentiate between highly differentiated liposarcoma (+), dedifferentiated liposarcoma (+) and myxoid liposarcoma, pleomorphic liposarcoma, spindle lipoma, pleomorphic lipoma and other high-grade sarcomas.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment corresponding to N-terminal of human CDK4 protein

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for CDK4

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Alternative Name: CDK4

Background:

CDK4 protein, CDK4_HUMAN, Cell division protein kinase 4, CMM3, Crk3, Cyclin dependent kinase 4, Melanoma cutaneous malignant 3, MGC14458, p34 cdk4, PSK-J3

MCF7, HEK293T, cervical carcinoma and liposarcomas.|Human urothelial carcinoma, K-562

Molecular Weight: 34kDa

Gene ID: 1019, 95577

UniProt: P11802

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, Regulation of Cell Size