Recombinant Smooth Muscle Actin antibody

Catalog No. ABIN7863319

This Rabbit Monoclonal antibody specifically detects Smooth Muscle Actin in WB and IHC. It exhibits reactivity toward Human, Rat, Mouse, Rabbit and Guinea Pig.

Quick Overview for Recombinant Smooth Muscle Actin antibody (ABIN7863319)

Target: Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

Antibody Type: Recombinant Antibody

Reactivity: Human, Rat, Mouse, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Smooth Muscle Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: ACTA2-1614R

Product Details anti-Smooth Muscle Actin Antibody

Purpose: Recombinant Actin, Smooth Muscle (Leiomyosarcoma Marker) Antibody

Specificity: Actin is a major component of the cytoskeleton and is present in most cell types. It is highly specific to actin from smooth muscles. This MAb does not stain cardiac or skeletal muscle, however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human ACTA2 protein

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Smooth Muscle Actin

Target: Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

Alternative Name: Actin, Smooth Muscle

Background:

ACTA2, Actin Alpha 2 Smooth Muscle Aorta, Actin Vascular Smooth Muscle, ACTSA, ACTVS, Alpha Cardiac Actin, Alpha-actin-2, Aortic Smooth Muscle, ASMA, Cell Growth-inhibiting Gene 46 Protein, Growth Inhibiting Gene 46

Kidney, skeletal muscle, Human uterus or leiomyosarcoma, Mouse NIH/3T3 cells.

Molecular Weight: 42kDa

Gene ID: 59, 500483

UniProt: P62736

Pathways: Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development