Estrogen Receptor alpha antibody

Catalog No. ABIN7863752

The Mouse Monoclonal anti-Estrogen Receptor alpha antibody is suitable to detect Estrogen Receptor alpha in samples from Human, Mouse, Rat, Cow and Pig. It has been validated for IF and FACS.

Quick Overview for Estrogen Receptor alpha antibody (ABIN7863752)

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Reactivity: Human, Mouse, Rat, Cow, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This Estrogen Receptor alpha antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: AER311

Product Details anti-Estrogen Receptor alpha Antibody

Purpose: Estrogen Receptor (Marker of Estrogen Dependence) Antibody

Specificity: This MAb is specific to ER alpha and shows minimal cross-reaction with other members of the family. Epitope of this MAb is mapped between aa495-595 of E/F domain. ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: ER purified from cow uterus

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Estrogen Receptor alpha

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Alternative Name: Estrogen Receptor

Background:

Estrogen Receptor alpha delta 4*5,6,7*/654 isoform, Estrogen Receptor alpha delta 4 +49 isoform, Nuclear receptor subfamily 3 group A member 1

MCF-7 Cells or Breast Carcinomas

Molecular Weight: ~67kDa

Gene ID: 2099, 208124

UniProt: P03372

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly