Estrogen Receptor alpha antibody

Catalog No. ABIN7861282

The Mouse Monoclonal anti-Estrogen Receptor alpha antibody (Clone AER311) (ABIN7861282) specifically detects Estrogen Receptor alpha in IF and FACS. The antibody is reactive with Human, Mouse, Rat, Cow and Pig samples.

Quick Overview for Estrogen Receptor alpha antibody (ABIN7861282)

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Reactivity: Human, Mouse, Rat, Cow, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This Estrogen Receptor alpha antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: AER311

Product Details anti-Estrogen Receptor alpha Antibody

Purpose: Estrogen Receptor (Marker of Estrogen Dependence) Antibody

Specificity: This MAb is specific to ER alpha and shows minimal cross-reaction with other members of the family. Epitope of this MAb is mapped between aa495-595 of E/F domain. ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: ER purified from cow uterus

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Estrogen Receptor alpha

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Alternative Name: Estrogen Receptor

Background:

Estrogen Receptor alpha delta 4*5,6,7*/654 isoform, Estrogen Receptor alpha delta 4 +49 isoform, Nuclear receptor subfamily 3 group A member 1

MCF-7 Cells or Breast Carcinomas

Molecular Weight: ~67kDa

Gene ID: 2099, 208124

UniProt: P03372

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly