IL-1 beta antibody

Catalog No. ABIN7861546

The Mouse Monoclonal anti-IL-1 beta antibody (Clone IL1B-463) (ABIN7861546) specifically detects IL-1 beta in IHC and ELISA. The antibody is reactive with Human samples.

Quick Overview for IL-1 beta antibody (ABIN7861546)

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-1 beta antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Clone: IL1B-463

Product Details anti-IL-1 beta Antibody

Purpose: Interleukin-1 beta (IL-1 beta) Antibody

Specificity: Recognizes a protein of 17-31 kDa, identified as interleukin 1beta. It shows no cross-reaction with interleukin 1alpha. Epitopes of MAb IL1B/463 and IL1B/463 are different and provide a good pair for developing an ELISA. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human IL1B protein

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for IL-1 beta

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Alternative Name: Interleukin-1 beta

Background:

Catabolin, Hematopoietin 1 (H1), IFN beta inducing factor, IL-1 beta, IL1B, IL1F2, Interleukin 1 beta precursor, Interleukin-1 beta, LAF, Osteoclast activating factor (OAF), Preinterleukin 1 beta, Pro interleukin 1 beta

colon or liver tissue.|LPS stimulated human peripheral blood mononuclear cells. Human adrenal gland

Molecular Weight: 31kDa (proprotein), 17kDa (mature)

Gene ID: 3553, 126256

Pathways: NF-kappaB Signaling, Interferon-gamma Pathway, TLR Signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome