Androgen Receptor antibody (pTyr363)

Catalog No. ABIN7862504

This Rabbit Polyclonal antibody specifically detects Androgen Receptor in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Androgen Receptor antibody (pTyr363) (ABIN7862504)

Target: Androgen Receptor (AR)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Androgen Receptor antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Androgen Receptor Antibody

Binding Specificity: pTyr363

Purpose: Rabbit Anti-AR (phospho Tyr363) Polyclonal Antibody

Specificity: Phospho-AR (Y363) Polyclonal Antibody detects endogenous levels of AR protein only when phosphorylated at Y363.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human AR Phospho-Tyr363

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:10000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Androgen Receptor

Target: Androgen Receptor (AR)

Alternative Name: AR

Background: AR, DHTR, NR3C4, Androgen receptor, Dihydrotestosterone receptor, Nuclear receptor subfamily 3 group C member 4The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. AR contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract from the normal 9-34 repeats to the pathogenic 38-62 repeats causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described.

Molecular Weight: 85kD

Gene ID: 367

UniProt: P10275

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Chromatin Binding