Insulin antibody

Catalog No. ABIN7865657

This Mouse Monoclonal antibody specifically detects Insulin in IHC, ELISA, IF, FACS and ICC. It exhibits reactivity toward Human, Mouse, Pig, Cow and Rat.

Quick Overview for Insulin antibody (ABIN7865657)

Target: Insulin (INS)

Reactivity: Human, Mouse, Pig, Cow, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Insulin antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: 2D11-H5

Product Details anti-Insulin Antibody

Purpose: anti-Insulin mouse monoclonal, 2D11-H5, purified

Purification: affinity chromatography

Immunogen: purified pig insulin, conjugated to KLH

Isotype: IgG1 kappa

Application Details

Application Notes:

ICC/IF_dilution: 1:100-1:200 (0.5-1.0 μg/mL)

FACS_dilution: 0.5-1.0 μg/million cells in 0.1 ml

IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

elisa_dilution: assay dependent

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: store at 2 - 8 °C

Target Details for Insulin

Target: Insulin (INS)

Alternative Name: Insulin

Background: Insulin is a protein consisting of an α-chain of 21 amino acids and a β-chain of 30 amino acids and produced in the β-cells of the pancreas. 2D11-H5 is a specific insulin antibody as tested by ELISA and on human pancreatic tissue. Positive Control: MIA, PaCa-2 cells, pancreas or insulinoma.

Pathways: NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation