Integrin beta 2 antibody

Catalog No. ABIN7865662

The Mouse Monoclonal anti-Integrin beta 2 antibody (Clone 68-5A5) (ABIN7865662) specifically detects Integrin beta 2 in FACS, WB, IHC, IF and ICC. The antibody is reactive with Human samples.

Quick Overview for Integrin beta 2 antibody (ABIN7865662)

Target: Integrin beta 2 (ITGB2)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Integrin beta 2 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 68-5A5

Product Details anti-Integrin beta 2 Antibody

Purpose: anti-CD18 mouse monoclonal, 68-5A5, purified

Purification: affinity chromatography

Immunogen: stimulated human leucocytes

Isotype: IgG2a kappa

Application Details

Application Notes:

ICC/IF_dilution: 1:100-1:200 (0.5-1.0 μg/mL)

FACS_dilution: 0.5-1.0 μg/million cells in 0.1 ml

WB_dilution: 1:50-1:100 (1-2 μg/mL)

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: store at 2 - 8 °C

Target Details for Integrin beta 2

Target: Integrin beta 2 (ITGB2)

Alternative Name: CD18

Background: It recognizes a transmembrane glycoprotein of 95 kDa, identified as CD18 or intergrin-2 (Workshop III). It complexes non-covalently with either L, M, or X integrin (CD11a, b, or c) to form the heterodimers. LFA-1, MAC-1, and p150,95, respectively. LFA-1 is the receptor for three members of the Ig supergene family of proteins, ICAM-1 (CD54, ICCAM-2 (CD102), and Mac-1 and p150,95 bind to ICAM-1, fibrinogen, and IC3b. ICAM-3 (CD50). CD18/CD11 heterodimeric molecules are involved with cell/cell and cell/extracellular adhesion in immune and inflammatory responses. This Mab blocks these cellular interactions. 68-5A5 was clustered at the IIIrd International Workshop on human leucocyte differentiation antigens. Positive control: Human PBL and tonsil.

Pathways: NF-kappaB Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation